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Protocols for heat-stabilized tissue samples

After heat stabilization, it is important to follow an optimal protocol to maximize recovery of the proteins and peptides of interest and ensure that the final extracts are in a suitable condition for further analysis.

Based on collaborations with users of the Stabilizor system, as well as our in-house research and development, we continually review and optimize protocols to ensure the best possible results downstream.

Protocol for peptide extraction for LC-MS is included in the Stabilizor Peptide Extraction kit.

Recommended protocol: SDS-PAGE & Western blot

Protocol for protein extraction for 2D-GE is included in the Stabilizor 2D-GE Extraction kit.

Recommended protocol: Shot gun proteomics with LC-MS

Technical note: Extraction of proteins from heat-stabilized tissue samples

 

MSDS

Stabilizor 2D-GE Extraction Kit

MSDS - Diluent

Stabilizor 2D-GE Extraction Kit

MSDS - Dry component

Stabilizor Peptide Extraction Kit

MSDS - Extraction fluid

Stabilizor 2D-GE Extraction Kit

MSDS - DTT

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