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In the publication “Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues”, researchers from Prof. Jesper Olsen’s group at the NNF Center for Protein Research at the University of Copenhagen, describes an efficient method for tissue protein extraction in combination with phosphopeptide enrichment and high-resolution tandem MS. The method makes it is possible to quantify an impressive amount of phosphopeptides and protein phosphorylation sites from tissue samples in a limited time period. This work provides a promising platform for standardizing screening of tissue phosphoproteomes and leads the way for forthcoming studies.
Online Publication from Nature Communications: