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Extract endogenous peptides
Maximize recovery from tissue samples prior to LC-MS
Used together with the Stabilizor system, the StabilizorTM Peptide Extraction Kit facilitates differentiation and identification of endogenous peptides from degradative peptide fragments. The kit contains all necessary accessories and solutions to homogenize and extract endogenous peptides prior to LC-MS. A fully tested protocol aims to maximize recovery.
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Optimize recovery of water-soluble endogenous peptides
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Follow a ready to use, validated protocol - no need for optimization
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Produce high quality samples for consistent LC-MS analysis
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Reduce time to results
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Use standard laboratory equipment
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Efficient recovery of neuropeptides from heat-stabilized tissue
Neuropeptides that are produced, or have their action, within the central nervous system (CNS) are typical endogenous peptides, originating from neuropeptide-precursors stored in cellular vesicles. These precursors are cleaved or modified by enzymes before being released outside the cell as signals.
Recent publication
Results from a recent publication (Neuropeptide profiling of the bovine hypothalamus: Thermal stabilization is an effective tool in inhibiting post-mortem degradation. Michelle L. Colgrave, et al., Proteomics 2011, 11, Issue 7, pages 1264–1276) demonstrate an increased recovery of neuropeptides from tissue samples when utilizing heat stabilization followed by an optimal extraction protocol.
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