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The smallest changes upstream influence results downstream.
Defining the role of proteins as drugs, drug targets or disease biomarkers is crucial to the development of new therapeutics and our understanding of disease mechanisms. Yet changes to protein - and peptide profiles begin within seconds of a tissue being sampled. Vital information about the original protein profile may be destroyed or distorted, leading to inter-sample variation, risk of incorrect data interpretation and potentially misleading conclusions.
Conventional approaches to inhibiting these changes, such as using inhibitor cocktails, pH changes or cross-linking, may be reversible, involve the use of toxic compounds and interfere with downstream analytical techniques.
Rapid heat stabilization preserves protein profiles by instantly and permanently denaturing the proteolytic enzymes responsible for rapid biological changes.
Instant thermal denaturation
Post mortem changes of proteins and peptides begin within seconds of a biological sample being removed from its normal environment. Vital information about the original biological state may be destroyed or distorted, leading to inter-sample variation and the risk of incorrect data interpretation.
Denator's proprietary, patent pending, heat-stabilization technology utilizes conductive heating, at controlled pressure, to generate a rapid, homogenous thermal denaturation of proteins. This results in complete and permanent inactivation of all enzymes that could cause changes to a sample of tissue or fluid.
An automated process - optimal conditions for each sample
The Stabilizor system enables samples to be heat stabilized from the moment of sampling, preserving the original composition and state of proteins, peptides and post-translational modifications while increasing the accuracy and quality of analytical results.
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Made from inert materials to eliminate the risk of contamination, the cards are designed to enable subsequent transport and storage of the heat-stabilized samples.
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Maintainor Tissue - heat stabilize, transport and store tissue samples
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The fully automated procedure ensures consistent, reproducible treatment of samples. Sample size is determined using a highly accurate laser. Size, together with information about the physical state of the sample, for example fresh or frozen, is fed into algorithms of pre-programmed method templates. These algorithms determine the exact amount of heat required to ensure complete denaturation (the treatment time) of the proteins in the sample.
