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Stabilize biological samples
Heat stabilization permanently inhibits enzymatic activity
- Reveal phosphorylation states and discover new endogenous peptides
- Get closer to original in vivo profiles
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Prepare, analyze and re-analyze without risk of degradation
- minimize inter-sample variation
- improve data interpretation
The StabilizorTM system uses rapid heating to preserve tissue samples as close as possible to their in vivo state. Heat stabilization permanently prevents post-mortem changes from the moment of sampling and thereby increases the accuracy and quality of analytical results.
Using the Stabilizor system to heat-stabilize samples:
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Eliminates enzyme activity from the moment of sampling
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Stops degradation permanently - without using additives
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Standardizes sample handling - to improve reproducibility of analytical workflows
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Links upstream processing to downstream results - traceable treatment parameters
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Facilitates accurate sample comparison and data interpretation downstream
Inihibition of enzymatic activity to preserve phosphorylation states
Heat stabilization permanently inactivates phosphatases
Phosphatase activity measured using SensoLyte pNPP phosphatase kit (Anaspec).
Heat-stabilized mouse brain cortex samples show a significant lower level of phosphatase activity compared to snap frozen samples with and without inhibitors. Phosphatase activity is equivalent to background levels (Svensson et al. Heat stabilization of the Tissue Proteome: A New Technology for Improved Proteomics. J Proteome Res. 2009)
Biological samples successfully analyzed
The range of biological samples that have been heat-stabilized and analyzed successfully is expanding steadily as more scientists implement the Stabilizor system into their workflows. Currently, the benefits of heat-stabilization have been utilized when analyzing proteins or peptides in the following biological samples:
Proteins in brain, liver, heart, pancreas and breast tumor, erythrocytes and yeast.
Peptides in brain, thymus and pancreas.
Analytical techniques have included mass spectrometry, Western Blot with phospho-specific antibodies, 1D and 2D gels with phospho-specific stains and reversed phased protein arrays (RPPA)
Ordering information
Please contact your local Denator representative.
