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Identification of proteins, Quantitative proteomics, enzyme activity

Proteins indicating the risk of post-mortem change

A number of specific proteins, regardless of tissue or species, are often found expressed differentially in disease states, bringing into question the significance of these proteins as potential biomarkers. An overlap between these proteins commonly identified as changing in 2D-GE protein studies and those found to change post-mortem has also been found.

The list below is a compilation of proteins frequently found to appear in 2D-GE experiments which may indicate post-mortem alterations of the sample.

HGNC ID

12849

12852

12854

12855

4907

118

129

132

144

399

404

414

537

542

543

600

8822

830

2157

1455

9390

1373

2529

16812

2062

18304

1874

2770

2911

3014

2715

2972

28670

3208

10830

3350

3214

1491

3700

7242

4235

15987

4638

4141

4162

4212

5246

5261

5241

4827

5044

5244

5238

5384

6446

6636

6541

122

7220

7567

7584

7583

2716

7715

7850

15912

9253

9352

9353

16753

8896

8888

8548

8912

9568

4606

9702

8806

9021

678

11244

11162

11262

6510

11179

16987

11494

11132

11655

12437

22282

11619

4396

12405

12009

12012

11948

20778

12022

12513

12692

12665

12672

HGNC Symb.

YWHAB

YWHAG

YWHAQ

YWHAZ

HIBADH

ACO2

ACT1

ACTB

ACTG1

ALB

ALDH2

ALDOA

ANXA2

ANXA4

ANXA5

APOA1

PEA15

ATP5B

CNN3

CALR

PRKAR1B

CA2

CTSD

ADCK3

CLIC1

COTL1

CFL1

DES

DLST

DPYSL2

DDAH1

DNM1

EFHD2

EEF1B2

SH3GL1

ENO1

EEF2

CAPZB

FH

NAPG

GFAP

GLRX3

GSTP1

GAPDH

GARS

GDA

SHPB1

HSPD1

HSPA8

HBB

HNRNPK

HSPA9

HSPA5

IDH3A

KRT8

LMNA

LDHB

Acp1

MPP2

MYH1

MYL3

MYL2

DDAH2

NDUFS8

NME2

NSFL1C

PPIA

PRDX1

PRDX2

PRDX6

PGK1

PGAM1

P4HB

PHB

PSME1

PDIA3

PURB

PDHA1

PKM2

ARHGDIA

SPINK1

SNRPF

SPRR2B

STMN1

SOD1

Sugt1

SYN1

Snap25

TCP1

TXNRD1

Tceal5

Tceab2

Gnb1

TTR

TPI1

TPM3

TNNT1

TUBB

TPT1

Uchl1

VIM

VCL

VDAC2

 

Protein name

14-3-3 Protein beta/alpha

14-3-3 Protein gamma

14-3-3 Protein theta

14-3-3 Protein zeta/delta

3-hydroxyisobutyrate dehydrogenase

Aconitase 2;mitocondrial

Actin, alfa

Actin, beta

Actin, gamma 1

Albumin

Aldehyde dehydrogenase 2 family (mitochondrial)

Aldolase A, fructose-bisphosphate

Annexin A2

Annexin A4

Annexin A5

Apolipoprotein A1

Astrocytic phosphoprotein PEA-15

ATP synthase, H1transporting, beta

Calponin-3

Calreticulin

cAMP-dependent protein kinase type I-beta

Carbonic anhydras 2

Cathepsin D

Chaperone ABC 1-like

Chloride intracellular channel 1

Coactosin-like 1

Cofilin 1 (nonmuscle)

Desmin

Dihydrolipoamide succinyltransferase

Dihydropyrimidinase-like protein 2

Dimethylarginine dimethylaminohydrolase

Dynamin

EF hand domain containing protein

Elongation factor 1-beta

Endophilin-A

Enolase 1

Eukaryotic translation elongation factor

F-actin-capping protein subunit beta

Fumarate hydratase

Gamma-soluble NSF attachment protein

Glial fibrillary acidic protein

Glutaredoxin-3

Glutathione S-transferase pi

Glyceraldehyde-3-phosphatedehydrogenase

Glycyl-tRNA synthetas

Guanine deaminase

Heat shock 27 kDa protein 1

Heat shock 60 kDa protein 1 (chaperonin)

Heat shock 70 kDa protein 8

Hemoglobin subunit beta-1

Heterogeneous nuclear ribonucleoprotein K

HSP70KDa protein (Mortalin)

HSP70kDa protein 5 (glucose-regulated protein, 78 kDa)

Isocitrate dehydrogenase [NAD] subunit alpha

Keratin 8

Lamin A/C

L-lactate dehydrogenase B chain

LMW phosphotyrosine protein phosphatase

MAGUK p55 subfamily member 2

Myosin heavy chain smooth muscle

Myosin light chain 3

Myosin regulatory light chain 2

N(G),N(G)-dimethylarginine dimethylaminohydrolase 2

NADH-ubiquinone oxidoreductase 23kDa subunit

Nonmetastatic cells 2 protein (NM23B)expressed in

NSFL cofactor protein p47

Peptidylprolyl isomerase A (cyclophilin A)

Peroxiredoxin 1

Peroxiredoxin 2

Peroxiredoxin 6

Phosphoglycerate kinase 1

Phosphoglycerate mutase

Procollagen-proline

Prohibitin

Proteasome (prosome,macropain) activatorsubunit 1

Protein disulfide isomerase family A, member 3

Purine-rich element binding protein B

Pyruvate dehydrogenase E1 alpha1

Pyruvate kinase, muscle

Rho GDP dissociation inhibitor (GDI) alpha

Serine protease inhibitor precursor

Small nuclear ribonuclear protein F

Small proline-rich protein 2B

Stathmin 1/oncoprotein 18

Superoxide dismutase 1 soluble

Suppressor of G2 allele of SKP1 homolog

Synapsin

Synaptosomal-associated protein 25

T-complex 1

Thioredoxin reductase 1

Transcription elongation A protein-like 5

Transcription elongation factor B polypeptide 2

Transducin beta chain

Transthyretin

Triosephosphate isomerase 1

Tropomyosin 3

Troponin T

Tubulin, beta

Tumour protein, translationally controlled 1

Ubiquitin thiolesterase

Vimentin

Vinculin

Voltage-dependent anion-selective channel protein 2

 

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