More endogenous peptides detected in mouse brain tissue compared to using standard protocol of methanol extraction on LTQ OrbiTrap MS. Data courtesy of Dr. Joel Cap-de-Ville, Sanofi-Aventis, France.
Peptidomics
Bioactive peptides fulfil important functions in the body and have the potential to be a rich source of disease biomarkers or drug molecules. The use of mass spectrometry-based methods to detect a large set of peptides in endocrine tissues is an efficient way to investigate the peptidome.
Rapid degradation in tissues, when removed from their natural environment, produces a large number of protein fragments that risk masking the peptides normally expressed in vivo expressed.
Using the Stabilizor T1 system to prevent sample degradation and preserve the in vivo peptide profile:
- Eliminates masking of in vivo expressed peptides
- Prevents degradation during sample processing
- Enables extraction of peptides in non-denaturing buffers without use of additives or precipitation steps
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“We discovered several novel, potentially bioactive, brain neuropeptides”
Prof. Per Andrén, Uppsala University, Sweden
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Our evaluation of this novel stabilization device, Stabilizor T1, has unequivocally demonstrated its’ utility in maintaining sample integrity in frozen hypothalamic tissue.”
Dr. Michelle Colgrave, CSIRO Livestock Industries, Australia
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"The results we got enabled us to find more endogenous peptides from less starting material compared to standard protocol of methanol extraction”
Dr. Joel Cap-de-Ville, Sanofi-Aventis, France
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“Using the Stabilizor system has really demonstrated the influence and importance of sample handling in both proteomics and peptidomics”
Dr. Birger Scholz, Uppsala University, Sweden
