Protease activity was measured using fluorescent protease kit (Pierce). Stabilized samples show a significant lower level of protease activity compared to snap frozen samples.

“Degradation begins seconds after sampling, thereby distorting the composition of the in vivo proteome. Denator’s rapid heat inactivation technology ensures complete elimination of enzymatic activity, thereby preserving the intact proteome. This will be an important tool that will help to further our understanding of the molecular basis of biological processes in health and disease.”
Prof. Michael Dunn, UCD Conway, Ireland

Proteomics

Understanding the role of proteins, as drugs, drug targets and disease biomarkers, is crucial for a wide range of serious diseases. Protein degradation, post sampling, can result in downstream analytical results reflecting a mixture of the in vivo proteome and its ex vivo degradation products. Analytical results may not reflect the true biological situation, resulting in potentially misleading conclusions.

Conventional ways of inhibiting protein degradation, such as using inhibitor cocktails, pH changes or cross-linking, have several disadvantages including reversibility, interference with downstream analysis, toxicity or impracticality.

Using the Stabilizor T1 system to prevent sample degradation and preserve the in vivo proteome:

Ensures that ex vivo degradation does not compromise downstream analysis
Avoids use of additives that may interfere with downstream analysis
Increases reproducibility
- Minimize inter-sample variation
- Improve data interpretation

The Stabilizor system has been used to preserve proteins prior to:

Mass spectrometry-based analysis
2D gel electrophoresis
Reversed phase protein arrays (RPPA)




	Protease activity was measured using fluorescent protease kit (Pierce). Stabilized samples show a significant lower level of protease activity compared to snap frozen samples. “Degradation begins seconds after sampling, thereby distorting the composition of the in vivo proteome. Denator’s rapid heat inactivation technology ensures complete elimination of enzymatic activity, thereby preserving the intact proteome. This will be an important tool that will help to further our understanding of the molecular basis of biological processes in health and disease.” Prof. Michael Dunn, UCD Conway, Ireland Proteomics Understanding the role of proteins, as drugs, drug targets and disease biomarkers, is crucial for a wide range of serious diseases. Protein degradation, post sampling, can result in downstream analytical results reflecting a mixture of the in vivo proteome and its ex vivo degradation products. Analytical results may not reflect the true biological situation, resulting in potentially misleading conclusions. Conventional ways of inhibiting protein degradation, such as using inhibitor cocktails, pH changes or cross-linking, have several disadvantages including reversibility, interference with downstream analysis, toxicity or impracticality. Using the Stabilizor T1 system to prevent sample degradation and preserve the in vivo proteome: Ensures that ex vivo degradation does not compromise downstream analysis Avoids use of additives that may interfere with downstream analysis Increases reproducibility Minimize inter-sample variation Improve data interpretation The Stabilizor system has been used to preserve proteins prior to: Mass spectrometry-based analysis 2D gel electrophoresis Reversed phase protein arrays (RPPA)

© Denator AB 2006-2009